Title: Thymosin beta(4) Administration Enhances Fracture Healing in Mice
Authors: Brady, RD
Grills, BL
Schuijers, JA
Ward, AR
Tonkin, BA
Walsh, NC
McDonald, SJ
Issue Year: 2014
Abstract Thymosin beta(4) (T beta(4)) is a regenerative peptide that we hypothesized would promote healing of fractured bone. Mice received a bilateral fibular osteotomy and were given i.p. injections of either T beta(4) (6 mg/kg) or saline. Calluses from saline-and T beta(4)-treated mice were analyzed for: (1) biomechanical properties and (2) composition using micro-computed tomography (mCT) and histomorphometry. Biomechanical analysis showed that T beta(4)-treated calluses had a 41% increase in peak force to failure (p<0.01) and were approximately 25% stiffer (p<0.05) than saline-treated controls. mCT analysis at 21 days post-fracture showed that the fractional volume of new mineralized tissue and new highly mineralized tissue were respectively 18% and 26% greater in calluses from T beta(4)-treated mice compared to controls (p<0.01; p<0.05, respectively). Histomorphometry complemented the mCT data; at 21 days post-fracture, T beta(4)-treated calluses were almost 23% smaller (p<0.05), had nearly 47% less old cortical bone (p<0.05) and had a 31% increase in new trabecular bone area/total callus area fraction compared with controls (p<0.05). Our finding of enhanced biomechanical properties of fractures in mice treated with T beta(4) provides novel evidence of the therapeutic potential of this peptide for treating bone fractures. (C) 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
URI: https://publications.svi.edu.au/publications/1803
Other Identifiers 10.1002/jor.22686
Publication type Article