Title: Facile preparation of tissue engineering scaffolds with pore size gradients using the muesli effect and their application to cell spheroid encapsulation
Authors: Forget, A
Rojas, D
Waibel, M
Pencko, D
Gunenthiran, S
Ninan, N
Loudovaris, T
Drogemuller, C
Coates, PT
Voelcker, NH
Blencowe, A
Issue Year: 2020
Publisher WILEY
Abstract Recent developments in stem cell biology have enabled the study of cell fate decisions in early human development that are impossible to study in vivo. However, understanding how development varies across individuals and, in particular, the influence of common genetic variants during this process has not been characterised. Here, we exploit human iPS cell lines from 125 donors, a pooled experimental design, and single-cell RNA-sequencing to study population variation of endoderm differentiation. We identify molecular markers that are predictive of differentiation efficiency of individual lines, and utilise heterogeneity in the genetic background across individuals to map hundreds of expression quantitative trait loci that influence expression dynamically during differentiation and across cellular contexts.
URI: https://publications.svi.edu.au/publications/7364
Other Identifiers 10.1002/jbm.b.34581
Publication type Article