Title: Interrogation of Phenotypic Plasticity between Epithelial and Mesenchymal States in Breast Cancer
Authors: Bhatia, S
Monkman, J
Blick, T
Pinto, C
Waltham, M
Nagaraj, SH
Thompson, EW
Issue Year: 2019
Publisher MDPI
Abstract Described is a simple, in vitro, dye dilution-based method for measuring antigen-specific CD4( )(+)T cell proliferation in human peripheral blood mononuclear cells (PBMCs). The development of stable, non-toxic, fluorescent dyes such as carboxyfluorescein succinimidyl ester (CFSE) allows for rare, antigen-specific T cells to be distinguished from bystanders by diminution in fluorescent staining, as detected by flow cytometry. This method has the following advantages over alternative approaches: (i) it is very sensitive to low-frequency T cells, (ii) no knowledge of the antigen or epitope is required, (iii) the phenotype of the responding cells can be analyzed, and (iv) viable, responding cells can be sorted and used for further analysis, such as T cell cloning.
URI: https://publications.svi.edu.au/publications/7821
Other Identifiers 10.3390/jcm8060893
Publication type Article