Title: Retinoic Acid Receptor gamma Activity in Mesenchymal Stem Cells Regulates Endochondral Bone, Angiogenesis, and B Lymphopoiesis
Authors: Green, AC
Rudolph-Stringer, V
Straszkowski, L
Tjin, G
Crimeen-Irwin, B
Walia, M
Martin, TJ
Sims, NA
Purton, LE
Issue Year: 2018
Publisher WILEY
Series J. Bone Miner. Res.:
Abstract Retinoic acid receptor (RAR) signaling regulates bone structure and hematopoiesis through intrinsic and extrinsic mechanisms. This study aimed to establish how early in the osteoblast lineage loss of RAR gamma (Rarg) disrupts the bone marrow microenvironment. Bone structure was analyzed by micro-computed tomography (mu CT) in Rarg(-/-) mice and mice with Rarg conditional deletion in Osterix-Cre-targeted osteoblast progenitors or Prrx1-Cre-targeted mesenchymal stem cells. Rarg(-/-) tibias exhibited less trabecular and cortical bone and impaired longitudinal and radial growth. The trabecular bone and longitudinal, but not radial, growth defects were recapitulated in Prrx1:Rarg(Delta/Delta) mice but not Osx1:Rarg(Delta/Delta) mice. Although both male and female Prrx1:Rarg(Delta/Delta) mice had low trabecular bone mass, males exhibited increased numbers of trabecular osteoclasts and Prrx1:Rarg(Delta/Delta) females had impaired mineral deposition. Both male and female Prrx1:Rarg(Delta/Delta) growth plates were narrower than controls and their epiphyses contained hypertrophic chondrocyte islands. Flow cytometry revealed that male Prrx1:Rarg(Delta/Delta) bone marrow exhibited elevated pro-B and pre-B lymphocyte numbers, accompanied by increased Cxcl12 expression in bone marrow cells. Prrx1:Rarg(Delta/Delta) bone marrow also had elevated megakaryocyte-derived Vegfa expression accompanied by smaller sinusoidal vessels. Thus, RAR gamma expression by Prrx1-Cre-targeted cells directly regulates endochondral bone formation and indirectly regulates tibial vascularization. Furthermore, RAR gamma expression by Prrx1-Cre-targeted cells extrinsically regulates osteoclastogenesis and B lymphopoiesis in male mice. (c) 2018 American Society for Bone and Mineral Research.
URI: https://publications.svi.edu.au/publications/4188
Other Identifiers 10.1002/jbmr.3558
Publication type Article