Title: Monoubiquitination by the human Fanconi anemia core complex clamps FANCI: FANCD2 on DNA in filamentous arrays
Authors: Tan, W
van Twest, S
Leis, A
Bythell-Douglas, R
Murphy, VJ
Sharp, M
Parker, MW
Crismani, W
Deans, AJ
Issue Year: 2020
Series ELIFE:
Abstract Dysregulation of lipid homeostasis is intimately associated with defects in insulin secretion, a key feature of type 2 diabetes. Here, we explore the role of the putative lipid transporter ABCA12 in regulating insulin secretion from beta-cells. Mice with beta-cell-specific deletion of Abca12 display impaired glucose-stimulated insulin secretion and eventual islet inflammation and beta-cell death. ABCA12's action in the pancreas is independent of changes in the abundance of two other cholesterol transporters, ABCA1 and ABCG1, or of changes in cellular cholesterol or ceramide content. Instead, loss of ABCA12 results in defects in the genesis and fusion of insulin secretory granules and increases in the abundance of lipid rafts at the cell membrane. These changes are associated with dysregulation of the small GTPase CDC42 and with decreased actin polymerisation. Our findings establish a new, pleiotropic role for ABCA12 in regulating pancreatic lipid homeostasis and insulin secretion.
URI: https://publications.svi.edu.au/publications/7320
Other Identifiers 10.7554/eLife.54128
Publication type Article