| Title: | Localization of dipeptidyl peptidase-4 (CD26) to human pancreatic ducts and islet alpha cells |
| Authors: | Augstein, P Naselli, G Loudovaris, T Hawthorne, WJ Campbell, P Bandala-Sanchez, E Rogers, K Heinke, P Thomas, HE Kay, TW Harrison, LC |
| Issue Year: | 2015 |
| Publisher | ELSEVIER IRELAND LTD |
| Series | Diabetes Res. Clin. Pract.: |
| Abstract | Aim: DPP-4/CD26 degrades the incretins GLP-1 and GIP. The localization of DPP-4 within the human pancreas is not well documented but is likely to be relevant for understanding incretin function. We aimed to define the cellular localization of DPP-4 in the human pancreas from cadaveric organ donors with and without diabetes. Methods: Pancreas was snap-frozen and immunoreactive DPP-4 detected in cryosections using the APAAP technique. For co-localization studies, pancreas sections were double-stained for DPP-4 and proinsulin or glucagon and scanned by confocal microscopy. Pan-creata were digested and cells in islets and in islet-depleted, duct-enriched digests analyzed for expression of DPP-4 and other markers by flow cytometry. Results: DPP-4 was expressed by pancreatic duct and islet cells. In pancreata from donors without diabetes or with type 2 diabetes, DPP-4-positive cells in islets had the same location and morphology as glucagon-positive cells, and the expression of DPP-4 and glucagon overlapped. In donors with type 1 diabetes, the majority of residual cells in islets were DPP-4-positive. Conclusion: In the human pancreas, DPP-4 expression is localized to duct and alpha cells. This finding is consistent with the view that DPP-4 regulates exposure to incretins of duct cells directly and of beta cells indirectly in a paracrine manner. (C) 2015 Elsevier Ireland Ltd. All rights reserved. |
| URI: | https://publications.svi.edu.au/publications/2077 |
| Other Identifiers | 10.1016/j.diabres.2015.10.010 |
| Publication type | Article |