Title: Transient Expression of AMPK Heterotrimer Complexes in Mammalian Cells
Authors: Oakhill, JS
Scott, JW
Dite, TA
Issue Year: 2018
Publisher HUMANA PRESS INC
Series Methods Mol. Biol.:
Abstract Regulation of AMP-activated protein kinase (AMPK) signalling is complex and involves contributions from adenine nucleotides, co-/posttranslational modifications, and isoform composition of the AMPK heterotrimer. It is becoming apparent that AMPK activation/inhibition by synthetic drugs involves similar levels of complexity. Major advances in our understanding of these mechanisms have been gained from recombinant expression systems that provide sufficient quantities of highly purified material for structure/function studies. Here, we provide a detailed protocol for transient expression of affinity-tagged AMPK complexes in mammalian cells. We have found this system to be optimal as a source of enzyme possessing regulatory modifications found in vivo.
URI: https://publications.svi.edu.au/publications/4710
ISSN
Other Identifiers 10.1007/978-1-4939-7598-3_10
Publication type Article