Title: Severe acute respiratory syndrome coronavirus 2 as a potential cause of type 1 diabetes facilitated by spike protein receptor binding domain attachment to human islet cells: An illustrative case study and experimental data
Authors: Venkatesh, N
Astbury, N
Thomas, MC
Rosado, CJ
Pappas, E
Krishnamurthy, B
MacIsaac, RJ
Kay, TWH
Thomas, HE
O’Neal, DN
Issue Year: 2021
Publisher WILEY
Abstract Aims Aim of this study is to report severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, responsible for coronavirus disease 2019 (COVID-19), as a possible cause for type 1 diabetes by providing an illustrative clinical case of a man aged 45 years presenting with antibody-negative diabetic ketoacidosis post-recovery from COVID-19 pneumonia and to explore the potential for SARS-CoV-2 to adhere to human islet cells. Methods Explanted human islet cells from three independent solid organ donors were incubated with the SARS-CoV-2 spike protein receptor biding domain (RBD) fused to a green fluorescent protein (GFP) or a control-GFP, with differential adherence established by flow cytometry. Results Flow cytometry revealed dose-dependent specific binding of RBD-GFP to islet cells when compared to control-GFP. Conclusions Although a causal basis remains to be established, our case and in vitro data highlight a potential mechanism by which SARS-CoV-2 infection may result in antibody-negative type 1 diabetes.
URI: https://publications.svi.edu.au/publications/8005
Other Identifiers 10.1111/dme.14608
Publication type Article