Title:	Effector-Memory T Cells Develop in Islets and Report Islet Pathology in Type 1 Diabetes
Authors:	Chee, J Ko, HJ Skowera, A Jhala, G Catterall, T Graham, KL Sutherland, RM Thomas, HE Lew, AM Peakman, M Kay, TWH Krishnamurthy, B
Issue Year:	2014
Publisher	AMER ASSOC IMMUNOLOGISTS
Series	JOURNAL OF IMMUNOLOGY: 192(2): 572-580
Abstract	CD8(+) T cells are critical in human type 1 diabetes and in the NOD mouse. In this study, we elucidated the natural history of islet-specific glucose-6-phosphatase catalytic subunit-related protein (IGRP)-specific CD8(+) T cells in NOD diabetes using MHC-tetramer technology. IGRP(206-214)-specific T cells in the peripheral lymphoid tissue increased with age, and their numbers correlated with insulitis progression. IGRP(206-214)-specific T cells in the peripheral lymphoid tissue expressed markers of chronic Ag stimulation, and their numbers were stable after diagnosis of diabetes, consistent with their memory phenotype. IGRP(206-214)-specific T cells in NOD mice expand, acquire the phenotype of effector-memory T cells in the islets, and emigrate to the peripheral lymphoid tissue. Our observations suggest that enumeration of effector-memory T cells of multiple autoantigen specificities in the periphery of type 1 diabetic subjects could be a reliable reporter for progression of islet pathology.
URI:	https://publications.svi.edu.au/publications/1746
Other Identifiers	10.4049/jimmunol.1302100
Publication type	Article
Grant ID	GNT0516700; GNT1037321; GNT1042735
Find it online	http://www.jimmunol.org/content/192/2/572.full.pdf+html